The Isolation of Rhodanese from Pseudomonas aeruginosa by Affinity Chromatography
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چکیده
منابع مشابه
The isolation of rhodanese from Pseudomonas aeruginosa by affinity chromatography.
Rhodanese (thiosulphate : cyanide sulphur-transferase; EC 2 . 8 . I . I) was originally ,described by Lang (1933). The enzyme catalyses the formation of thiocyanate from cyanide and thiosulphate according to the reaction : S20!+ CN-+ SCN+ SO:-. Rhodanese activity has been demonstrated in most mammalian tissues, with the greatest activity present in liver and kidney. The enzyme has been purified...
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Cyanide is a serious environmental pollutant and a biocontrol metabolite in plant growth-promoting Pseudomonas species. Here we report on the presence of multiple sulfurtransferases in the cyanogenic bacterium Pseudomonas aeruginosa PAO1 and investigate in detail RhdA, a thiosulfate:cyanide sulfurtransferase (rhodanese) which converts cyanide to less toxic thiocyanate. RhdA is a cytoplasmic enz...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-103-1-197